interactive crispr grna design tool Search Results


grna designer tools  (ATUM Bio)
90
ATUM Bio grna designer tools
Grna Designer Tools, supplied by ATUM Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
grna designer tools - by Bioz Stars, 2026-04
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crispr sgrna design tool  (Benchling Inc)
90
Benchling Inc crispr sgrna design tool
Crispr Sgrna Design Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr sgrna design tool/product/Benchling Inc
Average 90 stars, based on 1 article reviews
crispr sgrna design tool - by Bioz Stars, 2026-04
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gencrispr grna design tool  (GenScript corporation)
90
GenScript corporation gencrispr grna design tool
Gencrispr Grna Design Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gencrispr grna design tool/product/GenScript corporation
Average 90 stars, based on 1 article reviews
gencrispr grna design tool - by Bioz Stars, 2026-04
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crispr single grna design tool  (Synthego Inc)
90
Synthego Inc crispr single grna design tool
Screening and validation of <t>CRISPR/Cas9-mediated</t> STAR KO in MA-10 cells. ( A ) Western blot screening for STAR KO cell line following CRISPR/Cas9-mediated STAR KO and FACS. Samples in lane 5 (STARKO2) and lane 8 (STARKO1) show no STAR band and, therefore, are STAR KOs. ( B ) qRT-PCR analyses of STARKO1 and STARKO2 where Gapdh was used as the housekeeping gene. STAR 1, STAR 2, STAR 3, and STAR 4 are primers for various regions of the mouse Star gene. Data are shown as the mean ± SD ( n = 3). ** p < 0.01; *** p < 0.001. ( C ) BLAST DNA sequence of Star in STARKO1 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO1 (bottom). Highlighted amino acids represent sequence changes. ( D ) BLAST DNA sequence of Star in STARKO2 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO2 (bottom). Underlined amino acids represent sequence changes.
Crispr Single Grna Design Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr single grna design tool/product/Synthego Inc
Average 90 stars, based on 1 article reviews
crispr single grna design tool - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


Screening and validation of CRISPR/Cas9-mediated STAR KO in MA-10 cells. ( A ) Western blot screening for STAR KO cell line following CRISPR/Cas9-mediated STAR KO and FACS. Samples in lane 5 (STARKO2) and lane 8 (STARKO1) show no STAR band and, therefore, are STAR KOs. ( B ) qRT-PCR analyses of STARKO1 and STARKO2 where Gapdh was used as the housekeeping gene. STAR 1, STAR 2, STAR 3, and STAR 4 are primers for various regions of the mouse Star gene. Data are shown as the mean ± SD ( n = 3). ** p < 0.01; *** p < 0.001. ( C ) BLAST DNA sequence of Star in STARKO1 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO1 (bottom). Highlighted amino acids represent sequence changes. ( D ) BLAST DNA sequence of Star in STARKO2 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO2 (bottom). Underlined amino acids represent sequence changes.

Journal: International Journal of Molecular Sciences

Article Title: Role of Constitutive STAR in Leydig Cells

doi: 10.3390/ijms22042021

Figure Lengend Snippet: Screening and validation of CRISPR/Cas9-mediated STAR KO in MA-10 cells. ( A ) Western blot screening for STAR KO cell line following CRISPR/Cas9-mediated STAR KO and FACS. Samples in lane 5 (STARKO2) and lane 8 (STARKO1) show no STAR band and, therefore, are STAR KOs. ( B ) qRT-PCR analyses of STARKO1 and STARKO2 where Gapdh was used as the housekeeping gene. STAR 1, STAR 2, STAR 3, and STAR 4 are primers for various regions of the mouse Star gene. Data are shown as the mean ± SD ( n = 3). ** p < 0.01; *** p < 0.001. ( C ) BLAST DNA sequence of Star in STARKO1 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO1 (bottom). Highlighted amino acids represent sequence changes. ( D ) BLAST DNA sequence of Star in STARKO2 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO2 (bottom). Underlined amino acids represent sequence changes.

Article Snippet: Two sets of ss DNA oligonucleotides specifically targeting exon2 of Star were designed after using Synthego’s CRISPR single gRNA Design Tool ( https://www.synthego.com ).

Techniques: Biomarker Discovery, CRISPR, Western Blot, Quantitative RT-PCR, Sequencing